September 1, 2014

All-in-one bacmids: an efficient reverse genetics strategy for influenza A virus vaccines.
Chen H, Angel M, Li W, Finch C, Gonzalez AS, Sutton T, Santos J, Perez DR.
J Virol. 2014; 88:10013-25. doi: 10.1128/JVI.01468-14. Epub 2014 Jun 18.

This is a neat variant to classical influenza reverse genetics. All segments are on a single large plasmid – a bacmid (a larger than usual circular DNA molecule) – that results in more rapid recovery of virus.